mouse anti dog1 monoclonal antibody  (Boster Bio)

Journal: Therapeutics and Clinical Risk Management

Article Title: Small bowel gastrointestinal stromal tumor: a retrospective study of 32 cases at a single center and review of the literature

doi: 10.2147/TCRM.S167248

Figure Lengend Snippet: Ileal GISTs and immunohistochemistry. Notes: A 55-year-old male with an ileal GISTs presented with melena. ( A1 ) Axial plain CT image does not reveal the primary tumor due to polymorphous intestine. ( A2 ) Axial contrast-enhanced CT image shows a 1.3×1.8 cm 2 tumor (arrow) with marked enhancement in the arterial phase. ( A3 ) DBE shows the intraluminal component of the tumor with engorged vessels. ( A4 ) Gross appearance of the operative specimen shows the extraluminal component of the tumor (arrow). ( B1 ) Histopathology shows the tumor cells are composed of spindle cells with a low mitotic count (≤5 mitoses/50 HPFs; HE staining). Tumor cells are strongly positive for CD117 ( B2 ), CD34 ( B3 ), and DOG1 ( B4 ). Tumor cells are negative for SMA ( C1 ), which is positive in vascular wall. S-100 ( C2 ) and Desmin ( C3 ) are negative in tumor cells. Ki-67 ( C4 ) is positive in some of the tumor nuclei (global index 2%). (Original magnification: B1 ×100; B2 – C4 ×200). Abbreviations: GISTs, gastrointestinal stromal tumors; CT, computed tomography; DBE, double-balloon enteroscopy; HPFs, high-power fields; HE, hematoxylin and eosin; SMA, smooth muscle actin.

Article Snippet: The routine diagnostic immunohistochemistry technique used a panel of seven primary antibodies purchased from Boster Biological Technology (Wuhan, China), which included a mouse anti-CD117 monoclonal antibody used at 1:100 dilution, a rabbit anti-CD34 polyclonal antibody used at 1:200 dilution, a mouse anti-DOG1 monoclonal antibody used at 1:200 dilution, a mouse anti-SMA monoclonal antibody used at 1:150 dilution, a mouse anti-S100 monoclonal antibody used at 1:50 dilution, a mouse anti-desmin monoclonal antibody used at 1:100 dilution, and a rabbit anti-Ki-67 monoclonal antibody used at 1:100 dilution.

Techniques: Immunohistochemistry, Histopathology, Staining, Computed Tomography

Journal: PeerJ

Article Title: DOG1 is commonly expressed in pancreatic adenocarcinoma but unrelated to cancer aggressiveness

doi: 10.7717/peerj.11905

Figure Lengend Snippet: (A–D) DOG1 positive ductal adenocarcinomas with strong (A), moderate (B), focal moderate (C), and weak immunostaining of tumor cells (D). (A) and (C) also contain DOG1 negative normal ducts (arrow). (E & F) DOG1 negative cancers with diffuse (E) and periductal DOG1 (F) staining of stromal cells (F).

Article Snippet: Endogenous peroxidase activity was blocked with Dako Peroxidase Blocking Solution (#52023; Agilent, Santa Clara, CA, USA) for 10 min. Anti-DOG1 mouse monoclonal antibody MSVA-201M (MS Validated Antibodies, Hamburg, Germany) was applied at 37 °C, 60 min, at 1:150.

Techniques: Immunostaining, Staining

Journal: PeerJ

Article Title: DOG1 is commonly expressed in pancreatic adenocarcinoma but unrelated to cancer aggressiveness

doi: 10.7717/peerj.11905

Figure Lengend Snippet: DOG1 immunostaining in cancer cells and cancer phenotype.

Article Snippet: Endogenous peroxidase activity was blocked with Dako Peroxidase Blocking Solution (#52023; Agilent, Santa Clara, CA, USA) for 10 min. Anti-DOG1 mouse monoclonal antibody MSVA-201M (MS Validated Antibodies, Hamburg, Germany) was applied at 37 °C, 60 min, at 1:150.

Techniques: Immunostaining

Journal: PeerJ

Article Title: DOG1 is commonly expressed in pancreatic adenocarcinoma but unrelated to cancer aggressiveness

doi: 10.7717/peerj.11905

Figure Lengend Snippet: DOG1 immunostaining in stroma cells and cancer phenotype.

Article Snippet: Endogenous peroxidase activity was blocked with Dako Peroxidase Blocking Solution (#52023; Agilent, Santa Clara, CA, USA) for 10 min. Anti-DOG1 mouse monoclonal antibody MSVA-201M (MS Validated Antibodies, Hamburg, Germany) was applied at 37 °C, 60 min, at 1:150.

Techniques: Immunostaining

Journal: PeerJ

Article Title: DOG1 is commonly expressed in pancreatic adenocarcinoma but unrelated to cancer aggressiveness

doi: 10.7717/peerj.11905

Figure Lengend Snippet: DOG1 immunostaining and CD8 positivity.

Article Snippet: Endogenous peroxidase activity was blocked with Dako Peroxidase Blocking Solution (#52023; Agilent, Santa Clara, CA, USA) for 10 min. Anti-DOG1 mouse monoclonal antibody MSVA-201M (MS Validated Antibodies, Hamburg, Germany) was applied at 37 °C, 60 min, at 1:150.

Techniques: Immunostaining